Rna Extraction Kit 

Kit Contents (250 Reactions)

*Ethanol is not supplied with the kit

MDS Viral RNA Extraction Kit Quantity

Spin columns

Collection Tubes (2 ml)

250

500

Centrifugal Tubes (1.5 ml) 250

Buffer 1 1 X 50 ml

Buffer 2 1 X 25 ml

Buffer 3 1 X 25 ml

Buffer 4 1 X 75 ml

Wash Buffer 1 1 X 150 ml

Wash Buffer 2 1 X 150 ml

Packaging

Zip Pouch

Zip Pouch

Zip Pouch

1 x 50ml Bottle

1 x 50ml Bottle

1 x 50ml Bottle

2 x 50ml Bottle

3 x 50ml Bottle

3 x 50ml Bottle

Elution Buffer 1 X 25 ml 1 x 50ml Bottle

MDSTM Viral RNA Extraction Kit represent a well-established technology for general-use viral RNA preparation. The kit combines the

selective binding properties of a silica-based membrane with the speed of microspin and is highly suited for simultaneous processing of

multiple samples. The sample is first lysed under highly denaturing conditions to inactivate RNases and to ensure isolation of intact viral

RNA. Buffering conditions are then adjusted to provide optimum binding of the RNA to the membrane, and the sample is loaded onto the

spin column. The RNA binds to the membrane, and contaminants are efficiently washed away in 2 steps using 2 different wash buffers.

High quality RNA is eluted in a special RNase-free buffer, ready for direct use or safe storage. The purified RNA is free of protein, nucleases

and other contaminants and inhibitors. The special membrane guarantees extremely high recovery of pure, intact RNA in just 20 min

without the use of phenol/chloroform extraction or alcohol precipitation.